Nepal: Sampling has been completed in the leptospirosis project

Study design

Febrile patients presenting to hospitals and Primary Health Centres in Kaski district were recruited into a case-control study. At the time of recruitment acute stage blood samples were obtained from the patients for serological analysis.

A One Health team of public health and veterinary officers then visited each patient's household between three to ten weeks after his/her enrollment in the study. At the time of the house visit convalescent blood samples were collected from the patients and blood samples were collected from the domestic animals in the household (cattle, buffalo, goat, sheep, pig, dog) and from any trapped rodents.


Over a 20-week period between April and September 2013, two hundred and ninety five patients with acute febrile illness were enrolled in the study.

The table below shows the numbers of convalescent human samples and animal samples that were collected by the end of September, 2013.

Total number of house holds followed up: 241 (82% of recruited patients)



Human convalescent 238
Goat 176
Buffalo 92
Cattle 60
Dog 20
Cat 1
Rat 16


Successes and challenges

  • The biggest success of this project so far has been excellent collaboration between the government public health and livestock services offices.
  • Challenges have included disproportional patient enrollments in different health institutions, coupled with interference due to political strikes.